RevoluGen

RevoluGen is an innovative next generation life sciences tools company that has developed a patented spin-column based technology for extracting High Molecular Weight DNA (HMW-DNA). The Fire Monkey / Fire Flower kit extracts and purifies HMW-DNA from cells in record time. RevoluGen’s Fire Monkey/Fire Flower nucleic acid isolation and purification (NAIP) technology has an easier, faster workflow that enables long-read genomic assemblies. It extracts HMW-DNA with average DNA fragment lengths of over 100,000 base pairs and simultaneously depletes DNA fragments below 10,000 base pairs. As a one-hour spin-column process it is fast and requires only standard laboratory equipment and minimal training. Using the Fire Monkey NAIP kit to prepare a mammalian cell sample for sequencing in an Oxford Nanopore Technologies R9.4.1 / LSK109 / MinION run produced an N50 of 56kb, whilst generating a total throughput of 21Gb, 2.8Gb of which in 100kb+ reads. Using a Fire Monkey NAIP kit to prepare E.Coli bacterial cells for sequencing in an Oxford Nanopore Technologies R9.4.1 / LSK109 / MinION run produced a complete assembly with ~1000x coverage of the chromosome (Flye, Q7) as well as recovery of a ~130kb plasmid (Q7, ~1000x coverage) using LSK109. 50x coverage was achieved with reads >120kb. In addition, the Fire Flower protocol included in the combined Fire Monkey / Fire Flower kit can be used for size depletion to remove the bulk of the DNA fragments below 10kb on its own after any other isolation process has been used. 50 column Fire Monkey / Fire Flower is globally available through the MilliporeSigma with the 10 column kit available direct from www.revolugen.co.uk