About this course
This 3-day course is designed for individuals seeking to build essential, hands-on competencies in mammalian cell culture scale-up. We focus on providing a strong, practical foundation by blending critical theory with intensive laboratory sessions, making this ideal for anyone beginning a career in a modern bioprocessing environment.
The curriculum is centred on direct practical experience. You will learn to manage the complete workflow, including cell line maintenance, media preparation, and the rigorous application of aseptic technique.
Training culminates in operating a bench-scale stirred tank bioreactor (1.5L Livit Flex), where you will execute inoculation, manage process parameters, monitor cell viability, and implement contamination control strategies.
By completing the course, the attendees will be presented with a ‘Certificate of Applied Mammalian Cell Bioprocessing’ from IBioIC.
Learning outcomes
Upon completion of this course, you will be able to:
- Master core aseptic techniques to maintain sterility in all cell culture operations.
- Perform routine cell line maintenance, including passaging, cryopreservation, and thawing of cryovials.
- Prepare and set up a bench-scale bioreactor, including media preparation and sensor calibration.
- Inoculate the bioreactor and understand seed train expansion principles for scaling up cell numbers.
- Monitor and control critical parameters (pH, dissolved oxygen, temperature) to ensure optimal growth.
- Use cell counting methods, such as trypan blue exclusion, to determine viability and plot growth kinetics.
- Apply contamination control strategies and identify common biological contamination sources.
- Harvest cells effectively, separating them from the process medium.
Who will benefit?
Scientists, engineers, graduates, technical managers, operators, especially those in their early careers in fermentation and upstream bioprocessing.
Anyone who would like to gain an insight into the theoretical and practical aspects of bio-manufacturing.
