Super-resolution imaging can reveal biological detail that conventional confocal microscopy cannot resolve, but many workflows still force researchers to choose between resolution, speed, multicolor capability and quantitative confidence. Sequential acquisition can introduce temporal mismatches between channels, while detector saturation, noise and repeated setting adjustments can slow experiments and compromise valuable data.
This webinar explores how advances in image scanning microscopy support faster, simultaneous multicolor super-resolution imaging while maintaining quantitative accuracy. The session will examine how silicon photomultiplier technology combines sensitive detection, broad dynamic range and photon-resolving capability within a simultaneous multicolor super-resolution workflow.
The featured speakers will demonstrate how researchers can capture up to four super-resolution channels in one scan without switching between channels. By recording multiple biological events simultaneously, this approach helps preserve temporal relationships and ensure that colocalization data reflects the biology rather than instrument timing or channel-registration differences.
The session will also examine how advanced detector technology enables super-resolution imaging with a wide linear dynamic range and absolute photon quantitation comparable to those available in confocal acquisition. Faint subcellular signals and bright labels can be captured together without clipping, repeated gain adjustments or reacquisition. The featured speakers will discuss imaging approaches that achieve resolution down to 80 nm in XY and 200 nm in Z, while live-mode super-resolution imaging can maintain resolution down to 120 nm in XY and 350 nm in Z.
Application examples will illustrate how near-zero-noise cooled detection preserves signal-to-noise during high-speed scanning, supporting fast multicolor imaging of dynamic processes and larger samples. The session will address reproducibility through absolute photon counts, laser-power monitoring and system-performance checks, as well as how modular detector technology supports both super-resolution and standard confocal imaging workflows.
Register for this webinar to learn how super-resolution imaging improves multicolor acquisition and quantitative data reliability.
Who Should Attend
This webinar will appeal to Life Science Researchers, Imaging Scientists and Facility teams who use or evaluate confocal and super-resolution microscopy, including:
Cell and Molecular Biologists
Neuroscience and Developmental Biology Researchers
Cancer, Organoid and Drug Discovery Researchers
Live-cell, colocalization and quantitative imaging users
Microscopy Core Facility Managers and Imaging Specialists
Principal Investigators, Laboratory Managers and Technical decisionmakers
Researchers planning a new confocal system, adding super-resolution or upgrading an existing platform
What You Will Learn
Attendees will:
Discover how simultaneous acquisition of up to four super-resolution channels in one scan preserves temporal relationships and reduces sequential-channel mismatch
See how silicon photomultiplier technology combines wide linear dynamic range, low noise and photon-resolving capabilities to capture faint and bright signals
Learn how advanced imaging technologies achieve resolution down to 80 nm in XY and 200 nm in Z, and 120 nm in XY and 350 nm in Z during live-mode imaging
Explore how photon quantitation, laser-power monitoring and modular detector technology support reproducible confocal and super-resolution imaging
Register free on Xtalks:
https://xtalks.com/webinars/improve-data-quality-with-multicolor-super-resolution-imaging/
