About this course
This course is aimed at anyone who wants to develop an understanding of theory and techniques in protein purification using fast protein liquid chromatography (FPLC) on an ÄKTA chromatography system. The course, while specifically designed around the ÄKTA Avant system, provides comprehensive insights and practical knowledge that will be beneficial to all FPLC users, regardless of the specific model they are using. This training course will also focus on maintenance, cleaning and applications of using FPLC. The 3-day course consists of lectures, practical demos and hands-on exercises, hosted by the Industrial Biotechnology Innovation Centre (IBioIC). On completion of the course, the attendees will be presented with a certificate of course completion by IBioIC.
Learning outcomes
- Learn the basic principles and applications of different chromatography techniques, such as affinity, ion exchange, hydrophobic interaction, mixed mode and size exclusion
- Learn how a chromatography system works, how to prepare for a chromatography run, and importantly, how to ensure your chromatography system remains clean
- Purify green fluorescent protein (GFP) using Ion exchange chromatography (IEX) and hydrophobic interaction chromatography (HIC). Qualitative and quantitative analysis of purified protein using SDS-PAGE and UV-Vis spectroscopy
- Learn how to pack chromatography columns like a pro through a live demonstration and discover why it matters for your chromatography results
Who will benefit?
- Scientists, engineers, graduates, technical managers and operators, especially those in their early careers in downstream bioprocessing
- Anyone who would like to start using a chromatography system or wants to gain an understanding of the theory behind chromatography techniques
